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Genetic Mice

Genetic Mice

Transgenic Outline
Introduction 
Experimental Timeline 
Plan the Experiment 
Clone the Transgene 
Transgene Screening 
Transgene Expression 
DNA Microinjection 
Identify Transgenic Founders 
Southern Analysis 
Transgenic Analysis 
Pertinent Publications 
Review Articles and Books

1. Introduction. This is a brief outline of the steps necessary to obtain a transgenic mice. Simply put, the investigator constructs a transgene with a promoter and a structural gene (for example a reporter gene such as lacZ or a transcription factor). DNA is prepared and microinjected into fertilized mouse eggs. Potentially transgenic mice are born. Transgenic founders are identified and bred to produce offspring for analysis. Core personnel are available for consultation on all aspects of transgenic research. Contact Thom Saunders with any questions or Meet the Staff.

2. Plan the experiment. What is the purpose of your experiment? Do you want to analyze tissue specific regulatory sequences? Do you want to overexpress a protein in a specific cell lineage? You will need to obtain or clone the desired promoter and structural gene. Certain genes will be deleterious or incompatible with proper growth and development of the embryo, special arrangements should be made if you expect embryonic lethality from transgene expression. The

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