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A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ALL You Have Viewed Too Many Free Essays, Term Papers, and Book Reports. Spectrophotometer Analysis Spectrophotometer Analysis This experiment involved the use of a spectrophotometer and solution mixed by us in the laboratory. The spectrophotometer was used to analyze the speed of the reaction taking place in the sample through the use of light. A solution called guiacol was used because its reactivity with oxygen causes it to turn a brown color adding pigmentation to the solution. (Jacklett 2000) All the solutions were made using guiacol, turnip extract, H202 and dH20. The graphs show how the varying amounts of substrate and enzyme concentration affected the percentage of light absorbed by the solution in the spectrophotometer. Introduction This experiment was conducted in order to ascertain the effect of enzyme and substrate concentration on enzymatic reactions taking place in solutions we prepared. An enzyme is a polypeptide macromolecule that catalyzes biochemical reactions ( Bugg 1999). A spectrophotometer was used in order to detect the rate of light absorption by the solution. This number acted as a speedometer for the reaction. The quicker the solution got darker, the faster the reaction was going. When the spectrophotometer began giving off higher numbers, this indicated that the reaction was near its end because the solution was at one of its darkest points. All the tube, part, dh20, turnip, guiacol, extract, substrate, spectrophotometer, solution, enzyme, test, required, experiment, 01ml, solutions, reaction, consisted, h202, contained, amount, three, samples, peroxide, peroxidase, needed, made, light, data, concentration, 47ml, 10ml, 02ml, using, tubes, times, taking Word Count: 632 Acceptance_Essays American_History Anatomy_&_Physiology Animal_Science_&_Zoology Anthropology Architecture Art Astronomy Aviation Biographies Book_Reports Business Computers Creative_Writing Current_Events Economics Education Engineering English Environmental_Science Ethics European_History Film_&_TV Foreign_Languages Geography Government_&_Politics Health_&_Beauty History_Other Human_Sexuality Legal_Issues Marketing Mathematics Medicine Miscellaneous Music Mythology Philosophy Physics Poetry Political_Science Psychology Religion Science Shakespeare Social_Issues Sociology Speech Sports_&_Recreation Supernatural Technology Theater Theses_&_Dissertations

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